kato iii human gastric cancer cell lines  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Optimization of Helicobacter pylori Biofilm Formation in In Vitro Conditions Mimicking Stomach

doi: 10.3390/ijms25189839

Figure Lengend Snippet: Representative images showing a co-culture between H. pylori 2CML and KATO III cells growing on semi-permeable membranes in host-mimicking fluids. ( A ) A set of images obtained by fluorescence microscopy with different components of the co-culture model being stained (FM 1–43 to visualize bacterial biomass (biofilm), DAPI to show cell nuclei of eukaryotic cells, and WGA-conjugated Texas Red to indicate the presence of mucus glycoproteins (mucins)). Scale bars, 20 µm. ( B ) A set of images obtained by scanning electron microscopy. On the top, a control constituting non-infected KATO III cells; on the bottom, a co-infection of KATO III with H. pylori 2CML, where red arrows indicate areas of biofilm development. Scale bars, 20 µm.

Article Snippet: The human gastric cancer cell line KATO III (HTB-103) was obtained from the American Type Culture Collection and stored deep-frozen in the vapor phase of liquid nitrogen.

Techniques: Co-Culture Assay, Fluorescence, Microscopy, Staining, Electron Microscopy, Control, Infection

Journal: International Journal of Molecular Sciences

Article Title: Optimization of Helicobacter pylori Biofilm Formation in In Vitro Conditions Mimicking Stomach

doi: 10.3390/ijms25189839

Figure Lengend Snippet: Representative images showing a co-culture between H. pylori 2CML and KATO III cells growing in microfluidic conditions in host-mimicking fluids. ( A ) Time-dependent development of H. pylori biofilm attached to KATO III cells. ( B ) An image showing a one-day co-culture with a marking of specific components of this model, where red circles indicate KATO III cells and yellow dashed lines highlight areas of the development of H. pylori biofilm, being in close contact with these cells. Scale bars, 20 µm. To see stacked time-lapse sequences from the above experiments, please see .

Article Snippet: The human gastric cancer cell line KATO III (HTB-103) was obtained from the American Type Culture Collection and stored deep-frozen in the vapor phase of liquid nitrogen.

Techniques: Co-Culture Assay